The Turkish Journal of Pediatrics 2005 , Vol 47 , Num 3
Identification of an ancestral haplotype of the 35delG mutation in the GJB2 (connexin 26) gene responsible for autosomal recessive non-syndromic hearing loss in families from the Eastern Black Sea region in Turkey
1Department of Medical Biology, Hacettepe University Faculty of Medicine, Ankara, Turkey
2TÜBİTAK DNA/Cell Bank and Gene Research Laboratory, Hacettepe University Faculty of Medicine, Ankara,Turkey
3TÜBİTAK DNA/Cell Bank Audiology and Speech Pathology Unit, Hacettepe University Faculty of Medicine, Ankara,Turkey
4Department of Medical Biology, Karadeniz Technical University, Trabzon, Turkey
5Department of Ear, Nose and Throat, Hacettepe University Faculty of Medicine, Ankara,Turkey
Balcı B, Özbaş Gerçeker F, Aksoy S, Sennaroğlu G, Kalay E, Sennaroğlu L, Dinçer P. Identification of an ancestral haplotype of the 35delG mutation in the GJB2 (connexin 26) gene responsible for autosomal recessive non-syndromic hearing loss in families from the Eastern Black Sea region in Turkey. Turk J Pediatr 2005; 47: 213-221.

Mutations in the GJB2 gene have been shown to be the major cause of autosomal recessively inherited, prelingual, non-syndromic hearing loss. 35delG was found to be the most frequent mutation among Caucasians. In this study, we performed haplotype analysis of two large families with autosomal recessive non-syndromic hearing loss (totally 33 affected, 37 unaffected) from Trabzon (a city from the Eastern Black Sea region) by using polymorphic markers close to the 35delG mutation region, and identified a common haplotype, “2-6-4”. The frequency of the mutant chromosomes having the 2-6-4 haplotype was compared between the Eastern Black Sea region and the other regions of Turkey and the difference was found to be significant (χ2=5.13/df=1/p=0.023). Also, when the frequency of mutant and wild type chromosomes having the 2-6-4 haplotype was compared in the Eastern Black Sea region, a statistically significant difference was observed in the mutant chromosomes (χ2=7.46/df=1/p≤0.01). The results of this study demonstrate that the ancestral haplotype of the chromosomes bearing 35delG mutation in the Eastern Black Sea region is “2-6-4”. Keywords : prelingual, non-syndromic hearing loss, GJB2, 35delG, ancestral haplotype.

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