The Turkish Journal of Pediatrics
2010 , Vol 52 , Num 2
Association Between the Glu298Asp and T(-786)C Polymorphisms of the Endothelial Nitric Oxide Synthase Gene and Respiratory Distress in Preterm Neonates
Departments of 1Pediatrics, and 2Pharmacology, Hacettepe University Faculty of Medicine, Ankara, Turkey
Genetic polymorphisms in the gene that codes for endothelial nitric oxide
synthase (eNOS) have been associated with less nitric oxide availability and
with various cardiovascular diseases in humans. The objective of this study
was to analyze the genotype distributions and allele frequencies for the
Glu298Asp (G894T) and T(-786)C polymorphisms of the eNOS gene among
neonates with respiratory distress in comparison to healthy control subjects.
Fifty premature neonates with respiratory distress and 55 neonates without
any respiratory problem were included in the study. Genomic DNA from
all the neonates was analyzed by polymerase chain reaction. A polymerase
chain reaction–restriction fragment length polymorphism analysis of eNOS
gene polymorphisms was performed, and the results were compared. There
were no significant differences between the groups regarding either genotype
distributions or the allele frequencies for the Glu298Asp and T(-786)C
polymorphisms. These results suggest that eNOS Glu298Asp and T(-786)C
polymorphisms are not associated with development of respiratory distress.
Keywords :
endothelial nitric oxide synthase gene polymorphisms, preterm infant,
respiratory distress.